pbs based macsquant tyto running buffer (Miltenyi Biotec)

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Miltenyi Biotec pbs based macsquant tyto running buffer
Pbs Based Macsquant Tyto Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macsquant+tyto+running+buffer/MACSQuant+Tyto+Running+Buffer/pm42435424-303-31-38
Average 94 stars, based on 28 article reviews

pbs based macsquant tyto running buffer - by Bioz Stars, 2026-11

94/100 stars

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Incubation:

Article Title: CD133/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells
Article Snippet: .. The cells were then counted, centrifuged, and resuspended in MACSQuant Tyto Running Buffer (Miltenyi Biotec, #130-107-207) supplemented with CD133 antibody (1:10) and CD49a antibody (1:50) (100 μL buffer per 1 × 10 6 cells), and incubated for 15 min at room temperature. .. Subsequently, the cells were washed once with the Running Buffer and sorted on a Miltenyi MACSQuant Tyto sorter using gates to isolate single cells based on scatter characteristics.

Article Title: CD133/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells.
Article Snippet: .. The cells were then counted, centrifuged, and resuspended in MACSQuant Tyto Running Buffer (Miltenyi Biotec, #130-107-207) supplemented with CD133 antibody (1:10) and CD49a antibody (1:50) (100 μL buffer per 1 × 10 6 cells), and incubated for 15 min at room temperature. .. Subsequently, the cells were washed once with the Running Buffer and sorted on a Miltenyi MACSQuant Tyto sorter using gates to isolate single cells based on scatter characteristics.

Red Blood Cell Lysis:

Article Title: Treg activation during allograft tolerance induction requires mitochondrion-induced TGF- β 1 in type 1 conventional dendritic cells
Article Snippet: .. After RBC lysis and filtration, whole murine spleens were resuspended in 200 μL MACSQuant Tyto Running buffer (Miltenyi Biotec) and cells stained with MHCII, CD11c, XCR1, and CD172 antibodies for 20 minutes on ice in the dark. ..

Filtration:

Staining:

Article Title: IgG4 + plasma cell enrichment and λ-chain-biased BCR remodeling drive low-grade autoimmunity in chronic obstructive pulmonary disease
Article Snippet: For live B-cell sorting, PBMCs were stained with anti-CD45-FITC (BioLegend, clone HI30), anti-CD19-PerCP-Cy5.5 (BioLegend, clone HIB19) and fixable viability stain 506 (FVS506; BD Horizon) for 30 min on ice in the dark. .. Following staining, cells were washed twice and resuspended in MACSQuant Tyto running buffer (Miltenyi Biotec) at a concentration of 2–4 × 106 cells/mL. .. Cell sorting was performed using a MACSQuant Tyto cell sorter (Miltenyi Biotec) equipped with 405 nm, 488 nm, and 561 nm lasers and a closed cartridge-based system.

FACS:

Article Title: Cell-context response to germ layer differentiation signals is predetermined by the epigenome in regionalized epiblast populations.
Article Snippet: .. Cells were sorted into MACSQuant Tyto Running Buffer using the MACSQuant Tyto sorter (Miltenyi Biotec) or into ice-cold FACS buffer using the SONY SH800S cell sorter (Sony Biotechnology) at the DanStem Flow Cytometry Platform (University of Copenhagen, Copenhagen, Denmark). .. All reseeding experiments were performed using the MACSQuant Tyto sorter (Miltenyi Biotec), taking advantage of high-speed, multi-parameter flow sorting in the safety of a fully enclosed cartridge to prevent contamination.

Flow Cytometry:

Concentration Assay:

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction.
Article Snippet: The enriched cells were further labeled using MACS GMP clinical-grade antibodies: CD4-Vioblue (Clone M-T466, Miltenyi Biotec) and CD127-FITC (Clone MB15-18C9, Miltenyi Biotec) at 1:31 dilution and CD8APC (Clone BW135/80, Miltenyi Biotec) at either 1:31 or 1:21 dilution for 30 minutes at 4 °C, followed by a single wash with working buffer (300xg, 15 minutes). .. For sorting, the cells were resuspended in 10 mL of MACSQuant Tyto Running Buffer (Miltenyi Biotec) achieving an average concentration of 7.4 × 106 cells/mL (range: 6.9 × 106 to 7.8 × 106). .. The cells were filtered through a 20 μm cellstrainer and loaded into the MACSQuant Tyto high speed (HS) cartridges (Miltenyi Biotec) and sorted on the MACSQuant Tyto Cell Sorter (Miltenyi Biotec).

other:

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction
Article Snippet: The enriched cells were further labeled using MACS GMP clinical-grade antibodies: CD4-Vioblue (Clone M-T466, Miltenyi Biotec) and CD127-FITC (Clone MB15-18C9, Miltenyi Biotec) at 1:31 dilution and CD8-APC (Clone BW135/80, Miltenyi Biotec) at either 1:31 or 1:21 dilution for 30 minutes at 4 °C, followed by a single wash with working buffer (300xg, 15 minutes).

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